oxygen consumption rate measurement system  (NextGen Sciences)

Journal: The Journal of Biological Chemistry

Article Title: A CRISPR-edited isoform of the AMPK kinase LKB1 improves the response to cisplatin in A549 lung cancer cells

doi: 10.1016/j.jbc.2025.108308

Figure Lengend Snippet: Super LKB1 enhances the oxidative metabolism in A549-edited cells. A , Western blotting of HK2, LDHA, and GAPDH in cWT, c2SL+, and c3SL + cells; ( B ) normalized levels of proteins by GAPDH (a.u); ( C ) Western blotting of OXPHOS and GAPDH in cWT, c2SL+, and c3SL + cells. D , normalized levels of proteins by GAPDH (a.u); ( E ) oxygen consumption rate (OCR) by OROBOROS in cWT, c2SL+, and c3SL + cells; ( F ) comparison levels of O 2 in the cell lines in the basal condition and after treatments with oligomycin and FCCP. The data are presented as mean ± SD. Statistical analysis was performed by ANOVA followed by Dunnet’s test ∗ p < 0.05, ∗∗ p < 0.01. These data are representative of two independent experiments.

Article Snippet: The oxygen consumption rate measurement was taken at basal conditions, 1 mM oligomycin, and 1 mM FCCP in OROBOROS equipment (NextGen O2k).

Techniques: Western Blot, Comparison

Journal: Nature Communications

Article Title: Polyunsaturated fatty acids and p38-MAPK link metabolic reprogramming to cytoprotective gene expression during dietary restriction

doi: 10.1038/s41467-020-18690-4

Figure Lengend Snippet: a Knocking down drl-1 produces a DR-like phenotype in both WT as well as sek-1(km4) , resulting in depletion of stored fat to similar extent. Quantified data is provided in Supplementary Fig. . One of three independent experiments is shown. b Depletion of stored fat during DR, as seen in eat-2(ad465) , is independent of sek-1 . The sek-1 RNAi did not significantly change the low fat of eat-2(ad465) . Quantified data is provided in Supplementary Fig. . One of three independent experiments is shown. Multiple overlapping images were acquired at ×100 magnification to cover the entire worm body and stitched together to generate a contiguous image. c Knockdown of drl-1 decreased ROS levels to similar extent in WT and sek-1(km4) , as determined by DCFDA assay. Data are presented as mean values ± SEM. n = 4 independent experiments. Two-way ANOVA-Sidak’s multiple comparisons test, *** P = 0.0004, ** P = 0.0050. d Oxygen consumption rate (OCR) measured using Oroboros O2K shows similar respiratory rates in WT and sek-1(km4) . Data are presented as mean values ± SEM. n = 4 independent experiments. Unpaired two-tailed t test, ** P = 0.0011, *** P = 0.0006. e Autophagy, as determined by puncta formation in the seam cells of a LGG-1::GFP-expressing strain (upper panel), was increased in both WT as well as in sek-1(km4) when drl-1 was knocked down by RNAi. Representative images (upper panel) and quantification (lower panel) from one of two independent experiments shown. Experiments performed at 20 °C. n = 18 worms. Data are presented as mean values ± SEM. Two-way ANOVA-Sidak’s multiple comparisons test, **** P ≤ 0.0001. Scale bar = 10 μm. Experiments were performed at 20 °C. Source data are provided as a Source data file.

Article Snippet: Two-way ANOVA-Sidak’s multiple comparisons test, *** P = 0.0004, ** P = 0.0050. d Oxygen consumption rate (OCR) measured using Oroboros O2K shows similar respiratory rates in WT and sek-1(km4) .

Techniques: Two Tailed Test, Expressing